Journal: International Journal of Fertility & Sterility
Article Title: Extracellular Vesicles Present in Bone Marrow Mesenchymal Stromal/Stem Cell Conditioned Media Restore Spermatogenesis in Azoospermic Mice
doi: 10.22074/IJFS.2024.2021445.1619
Figure Lengend Snippet: Validation of MSCs and EVs derived from BMCM isolations was performed. The adherence and morphology of the cells were assessed, with A. BMMSCs and B. AT-MSCs. C. Differentiation into adipogenic lineages was observed for BM-MSCs and D. AT-MSCs, E. While osteogenic differentiation was confirmed for BM-MSCs and F. AT-MSCs. G. Chondrogenic differentiation was also achieved, as shown for BM-MSCs and H. AT-MSCs. I, J. Flow cytometry analysis revealed that both BM-MSCs and AT-MSCs expressed surface markers CD44 and CD105 at levels exceeding 95%, while hematopoietic markers CD34 and CD45 were expressed at levels below 10%. K. The isolation of EVs was confirmed using SEM and TEM, along with a detailed analysis of EV diameter distribution (scale bar A-H: 500 µm). MSCs; Mesenchymal stromal/stem cells, EVs; extracellular vesicles, BMCM; Bone marrow-derived mesenchymal stomal/stem cells conditioned medium, AT; Adipose tissue, SEM; Scanning electron microscope, and TEM; Transmission electron microscope.
Article Snippet: This procedure involved incubating 5×10 5 cells from the third passage with monoclonal antibodies specific to surface markers CD34, CD45, CD44, and CD105 (Jingmei Biotech Co. Ltd, Shenzhen, China), which were conjugated with fluorescein isothiocyanate (FITC) or phycoerythrin (PE) in 250 mL of phos-phate-buffered saline (PBS).
Techniques: Biomarker Discovery, Derivative Assay, Flow Cytometry, Isolation, Microscopy, Transmission Assay
